The electrophysiology unit has two separate systems for:
1) Patch-clamp and ion imaging experiments
This system integrates a patch-clamp amplifier and a stimulation/data acquisition system with an antivibration table, micromanipulators, a fluorescence microscope, a high sensitivity digital camera, and a high-speed wavelength switcher.
This system allows:
- Biophysical and pharmacological characterization of ion channels
- Analysis of calcium signaling by fluorescent (Fluo-4, Fura-2, GCAMP5-G, PH-PLC-GFP, ER-GECO1) and photosensitive (caged-IP3) probes
- Extracellular and intracellular pH measurements by BCECF and SNARF-1
2) Epithelial electrophysiology experiments
The unit has an 8-channel and a 4-channel transepithelial voltage/current-clamp system for simultaneous recordings of multiple epithelia in parallel.
These systems allow:
- Characterization of epithelial ion transport mechanisms in normal and pathological epithelia
- Evaluation of pharmacological agents to rescue the basic defect in cystic fibrosis (CF)
- Recordings using nasal epithelia of CF patients with rare orphan mutations to identify personalized treatments