Flow cytometry and Fluorescence-Activated Cell Sorting

Unit Manager: Stefano Pepe

Technician: Luigi Ferrante


Flow cytometry and FACS (fluorescence-activated cell sorting) are powerful technologies used for cell analysis and isolation in biomedical research. These technologies enable the identification of cells or specific cell subsets by measuring the physical and optical characteristics of single cells.

Flow cytometry analyzes a population of cells on a cell-by-cell basis, allowing researchers to locate and assess individual cells that may provide information on a disease state or biological process. This process has the capacity to simultaneously measure multiple parameters of single cells and is incredibly rapid, analysis rates can reach up to 10,000 cells per second.

Cell sorting uses principles of flow cytometry, allowing cells of interests to be identified according to a predetermined set of parameters. Laser technology detects information on cells and separates out those which align with the sorting parameters. Cell sorting and separation allows individual cellular populations to be collected, grown and studied in a highly selective manner.

The TIGEM FACS Unit is equipped with a benchtop analyzer (BD ACCURI C6) and a cell sorter (BD FACS ARIA III) supplied with 3 different lasers and an ACDU (Automated Cell Deposition Unit) module.

The TIGEM FACS Unit offers support and services for cell sorting and cell analysis.