Velia Siciliano, PhD
Synthetic and Systems biology lab for BiomedicineIstituto Italiano di Tecnologia-IIT, NapoliShort CVAbstract
Synthetic biology is a bioengineering discipline that aims at reprogramming cell fate by designing genetic circuits that sense and respond to disease biomarkers in the intracellular or extracellular environment. These smart interfaces process endogenous information and implement robust responses enabling high specificity and self-containment of desired output activation. Sensing cellular state may be particularly relevant when engineering T cell-based therapies for the treatment of solid tumors, to enhance the long-term efficacy of CAR-T cells. Over the talk I will discuss our current efforts for the design of genetic devices that can sense T cell state, focusing on strategies to enhance T cell persistence. Further, I will present our recent research on the impact of exogenous genetic payloads on mammalian cells and its implications for genetic circuits’ design.