Joep Beumer, PhD - "Modeling intestinal maturation and function in engineered human organoids"
- When Jul 08, 2024 from 12:00 PM to 01:15 PM (Europe/Berlin / UTC200)
- Where Tigem, Auditorium Angelo Maramai
- Contact Name Nicola Brunetti
- Contact Phone 081-19230659
-
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- https://www.tigem.it/newsroom/seminars/joep-beumer-phd-modeling-intestinal-maturation-and-function-in-engineered-human-organoids
- Joep Beumer, PhD - "Modeling intestinal maturation and function in engineered human organoids"
- 2024-07-08T12:00:00+02:00
- 2024-07-08T13:15:00+02:00
Joep Beumer, PhD
Institute of Human Biology (IHB)
Roche Pharma Research and Early Development
Basel, Switzerland
Short CV
Abstract
Intestinal stem cells at the bottom of crypts fuel the rapid renewal of the different cell types that constitute a multitasking tissue. The intestinal epithelium facilitates selective uptake of nutrients while acting as a barrier for hostile luminal contents. Recent studies revealed that these mature epithelial cell types display context-dependent functionality and can adapt to different requirements over their lifetime. We use intestinal organoid models as a reductionist approach to study the morphogens and transcriptional networks underlying the zonated functionalities along villi. By combining advanced fluorescent reporter models and single cell genomic approaches, we are reconstructing transcriptional networks guiding maturation of intestinal lineages. We built a versatile CRISPR-based platform for the introduction of single nucleotide variants and null mutants in intestinal organoids. We are employing these to perturb potential fate regulators and study disease-associated variants. I will further show how we use high-throughput genetic perturbation tools in organoids to study the role of oncogenic mutations.
Institute of Human Biology (IHB)
Roche Pharma Research and Early Development
Basel, Switzerland
Short CV
Abstract
Intestinal stem cells at the bottom of crypts fuel the rapid renewal of the different cell types that constitute a multitasking tissue. The intestinal epithelium facilitates selective uptake of nutrients while acting as a barrier for hostile luminal contents. Recent studies revealed that these mature epithelial cell types display context-dependent functionality and can adapt to different requirements over their lifetime. We use intestinal organoid models as a reductionist approach to study the morphogens and transcriptional networks underlying the zonated functionalities along villi. By combining advanced fluorescent reporter models and single cell genomic approaches, we are reconstructing transcriptional networks guiding maturation of intestinal lineages. We built a versatile CRISPR-based platform for the introduction of single nucleotide variants and null mutants in intestinal organoids. We are employing these to perturb potential fate regulators and study disease-associated variants. I will further show how we use high-throughput genetic perturbation tools in organoids to study the role of oncogenic mutations.