Diego di Bernardo
Full Professor in Biomedical Engineering , University of Naples “Federico II”
Coordinator of Genomic Medicine Program - (TIGEM)
Biomolecular engineering means to design and construct biomolecular gene circuits combining quantitative methods from Engineering with experimental techniques from Synthetic Biology. I will illustrate how we engineered biomolecular circuits for inducible control of gene expression, overcoming limitations of current approaches such as leakiness, small dynamic range and lack of robustness. I will show how these circuits can be used to engineer adeno-associated virus (AAV) producer cell lines that can generate high-titer and high-quality AAV vectors for gene therapy applications, overcoming current limitations of the triple-transfection method. I will also demonstrate how these biomolecular circuits can be used to create whole cell biosensors that can detect and report the presence of biomarkers or metabolites to monitor disease states, such as intracellular copper levels in vitro and in vivo model of Wilson’s disease.