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Luis Galietta

Principal Investigator, TIGEM
Head of Cell Culture and Cytogenetics Core, TIGEM

Associate Professor of Medical Genetics, Department of Translational Medicine, University of Naples "Federico II", Italy.

Pharmacological modulation of ion transport to treat the basic defect in cystic fibrosis and other genetic diseases

Cystic fibrosis (CF) is one of the most frequent genetic diseases, caused by mutations that impair the function of CFTR, a protein localized on the membrane of epithelial cells. The main role of CFTR is to mediate secretion of chloride and bicarbonate through the epithelia of different organs.  This process controls the hydration and pH of the fluids covering epithelial surface. Loss of CFTR function has consequences in multiple organs including lungs, pancreas, liver, sweat glands, and the reproductive system. However, the lung disease is a major cause of morbidity and mortality in CF.  The lack of CFTR impairs the innate natural defenses of the airways causing bacterial infection and mucus accumulation.  In many patients, the progressive loss of respiratory function in the last stage of the disease results into lung transplant as a life-saving intervention.

The basic defect in CF can be targeted with pharmacological strategies.  Depending on the type of mutations, CFTR activity can be restored with specific drugs called correctors and potentiators.  Such molecules act with different mechanisms.  Correctors improve the stability and folding of CFTR when it is affected by mutations like F508del, the most frequent among CF patients.  Instead, potentiators act as stimulators of CFTR and are therefore particularly effective for mutations that compromise the process of CFTR activation.

My research group has been particularly active in the search of effective correctors and potentiators to treat the basic defect in CF.  In collaboration with Prof. Alan S. Verkman (University of California San Francisco) we developed high-throughput methods that were subsequently used to screen large chemical libraries.  With this strategy, several active molecules were found.  High-throughput screening also allowed identification of potent and selective CFTR inhibitors that are important as tools of research and possibly useful as drugs for other genetic diseases like polycystic kidney disease. 

In collaboration with a research team led by Dr. Tiziano Bandiera at the Italian Institute of Technology (IIT), we are continuing our search for effective correctors and potentiators.  The project is supported by the Italian Cystic Fibrosis Foundation. By screening thousands of molecules and chemical modification of the resulting hits, we have found so far correctors with very high potency and efficacy on bronchial epithelial cells from CF patients.  The goal is to develop a promising preclinical drug candidate.

The basic defect in CF can be corrected by targeting other proteins.  In 2008, my research group identified TMEM16A as a second type of protein that, in addition to CFTR, mediates chloride and bicarbonate secretion in epithelial cells.  In theory, pharmacological stimulation of TMEM16A could circumvent defective CFTR, a strategy that could be important for those CF patients carrying mutation that are not sensitive to correctors and potentiators.  We are now working on TMEM16A to confirm its importance as a therapeutic target in CF and to find pharmacological activators.

Cell Culture Facility

The Cell Culture Facility provides fully-equipped cell culture facilities and technical support for cellular and cytogenetic studies to TIGEM investigators.

In the last years, research at TIGEM has confirmed that collection of cell lines from individuals with genetic disorders is essential in order to test candidate genes and to perform functional studies. These cell lines have been collected and organized into a database, which includes a wide variety of immortalized cell lines, as well as fibroblast and lymphoblast cell lines from patients with various genetic diseases. This collection has been useful in providing readily available sources of DNA/RNA for projects organized for the investigation of pathogenetic mechanisms.

The Core is responsible for the establishment, maintenance, and centralized database storage of these cell lines. In addition, the Core offers cytogenetic services such as chromosome analyses (standard and high resolution chromosome analysis) and fluorescence in situ hybridization (FISH) on cell lines, peripheral blood samples, embryonic stem cells, induced pluripotent stem (iPS) cells, as well as directly on tissue samples. Lastly, the Core provides mouse primary hepatocytes cultures and performs screening and treatment processes for detection and removal of Mycoplasma contamination, respectively.

Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, Pfeffer U, Ravazzolo R, Zegarra-Moran O, Galietta LJ (2008) TMEM16A, A membrane protein associated with calcium-dependent chloride channel activityScience 322: 590-594

Pedemonte N, Tomati V, Sondo E, Caci E, Millo E, Armirotti A, Damonte G, Zegarra-Moran O, Galietta LJ (2011) Dual activity of aminoarylthiazoles on the trafficking and gating defects of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel caused by cystic fibrosis mutations.  J Biol Chem 286:15215-15226

Scudieri P, Caci E, Bruno S, Ferrera L, Schiavon M, Sondo E, Tomati V, Gianotti A, Zegarra-Moran O, Pedemonte N, Rea F, Ravazzolo R, Galietta LJ (2012) Association of TMEM16A chloride channel overexpression with airway goblet cell metaplasia. J Physiol 590: 6141-6155

Pedemonte N, Galietta LJ (2014) Structure and function of TMEM16 proteins (anoctamins). Physiol Rev 94: 419-459

Caci E, Scudieri P, Di Carlo E, Morelli P, Bruno S, De Fino I, Bragonzi A, Gianotti A, Sondo E, Ferrera L, Palleschi A, Santambrogio L, Ravazzolo R, Galietta LJ (2015) Upregulation of TMEM16A protein in bronchial epithelial cells by bacterial pyocyanin.  PLoS One 10:e0131775

Luis Galietta MSc

Cell Biology and Disease Mechanisms

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