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Nuria Montserrat, PhD - "Engineering solutions for human pluripotent stem cells differentiation: from organoid derivation to disease modelling applications"

Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Pluripotency for Organ Regeneration, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Technology (BIST), Barcelona, and Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina, Madrid, Spain.
When Feb 18, 2020
from 12:00 PM to 01:15 PM
Where Tigem, Auditorium Vesuvius
Contact Name
Contact Phone 08119230659
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Abstract
The generation of human pluripotent stem cells (hPSCs) derived organoids is one of the biggest scientific advances in regenerative medicine. Recently, we have demonstrated that by lengthening the time that hPSCs are exposed to a three-dimensional microenvironment in the presence of defined renal inductive signals we are able to generate kidney organoids that transcriptomically match second-trimester human fetal kidneys. Furthermore, we have developed a transplantation method that exploits the intrinsic properties of the chick chorioallantoic membrane (CAM) to recreate a soft in vivo microenvironment for organoid growth and differentiation, including vascularization in vivo. Next, through bioengineering we have mimicked the stiffness of the chick CAM by fabricating compliant hydrogels. This approach resulted in the acceleration of kidney organoid formation proving that mechanical cues are determinant for the generation of hPSC-renal progenitor cells and kidney organoids. We will further discuss how these findings are pushing our research towards the application of different bioengineering strategies (i.e., including 3D bioprinting) for the generation of kidney organoids from hPSCs. Finally, we will also present recent findings when exploiting kidney organoid platform with CRISPR/Cas9 technology for applications in disease modeling and the study of morphogenesis.

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