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Jonathan Goodwin, Ph.D. - "Use of pooled CRISPR screening to understand selective autophagy"

Casma Therapeutics, Cambridge, MA
When Mar 27, 2018
from 11:00 AM to 12:30 PM
Where Tigem Auditorium "Vesuvius"
Contact Name
Contact Phone 08119230659
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Abstract
Selective autophagy refers to a process by which distinct cellular constituents are targeted to a growing autophagosome by a group of proteins known as autophagy cargo receptors. While the core machinery governing autophagosome biogenesis is often utilized across selective autophagy pathways, the regulators and input to each cargo receptor remains poorly defined. We have employed pooled CRISPR screening, both at genome-scale and with follow-on CRISPR minipools, to probe differential regulation of cargo receptors and influence of pathway activation state on accessory proteins. I will give an overview of the screening approach, some learnings from using reporters vs. endogenous readouts, and describe identification of core regulatory mechanisms of ferritin lysosomal turnover derived from a genome-wide approach.

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