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Brian Eames, PhD - "Using zebrafish genetics and 3D-bioprinting to dissect and treat osteoarthritis"

Associate Professor Skeletal Development & Evolution Dept. of Anatomy, Physiology, and Pharmacology University of Saskatchewan, Canada
When Jun 14, 2019
from 12:00 PM to 01:30 PM
Where Tigem, Vesuvius Auditorium
Contact Name
Contact Phone 081-19230659
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Abstract
Progression of osteoarthritis (a common disease disrupting articular cartilage) is poorly understood, and permanent therapies for osteoarthritis remain elusive.  To better understand molecular mechanisms underlying osteoarthritis progression, we carry out functional genetics in zebrafish.  A mutagenesis screen in zebrafish led to our focus on proteoglycan (PG) synthesis genes during skeletal development, since phenotypes of PG mutants mimic osteoarthritic tissues.  In addition to the known structural roles of PGs in the cartilage extracellular matrix, our work uncovered a biological role for cartilage PGs in regulating cartilage gene expression.  We're currently exploring the role of PG-dependent growth factor signalling in this process, as well as identifying additional PG sulfation genes that regulate PG function.  Complementing these projects are studies that use 3D-bioprinting to engineer articular cartilage as a permanent solution for osteoarthritis.  Independent fabrication of PCL and cell-impregnated hydrogel strands allows us to mimic the complex mechanical and biological properties of articular cartilage.  With the help of novel synchrotron imaging techniques, we have demonstrated some in vitro and in vivo (small animal) success of our unique tissue engineering approach.  We're currently examining long-term success of 3D-bioprinted articular cartilage in a pre-clinical large animal model

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