Angela Bachi, Ph.D - "Quantitative proteomics reveals the leukocyte specific protein Sp140 as a new component of repressive chromatin"
Jun 14, 2016
from 12:00 PM to 01:00 PM
|Where||Tigem Auditorium "Vesuvius"|
|Contact Name||Alessandro Fraldi|
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Curriculum Vitae: Dr.ssa Angela Bachi
Substantive position in applicant organisation: Senior Principal Investigator
Subject discipline(s): Proteomics, Biochemistry, Biotechnology
1990 Degree in Pharmaceutical Chemistry & Technology, University of Genova, Italy.
1991 Degree in Pharmacy, University of Genova, Italy.
1996 PhD in Biochemistry, Mario Negri Institute for Pharmacological Research, Milan, Italy.
1990-1992: Undergraduate student at the Pharmaceutical Analysis & Technology Department of the University of Genoa, Italy.
1993-1996: PhD student supported by the "Fondazione Lombardia per l'Ambiente". Laboratory of Environmental Pharmacology and Toxicology, Analytical Biochemistry Unit, Mario Negri Institute for Pharmacological Research, Milan, Italy.
1997-1999: Postdoctoral fellow in Matthias Mann and Matthias Wilm group, at the EMBL, Heidelberg, Germany.
2000-2002: junior Group Leader, Biological Mass Spectrometry Unit, Department of Molecular Biology and Functional Genomics, San Raffaele Scientific Institute, Milan, Italy.
2003- 2013: Head of Unit, Biomolecular Mass Spectrometry Unit, Division of Genetics & Cell Biology, San Raffaele Scientific Institute, Milan, Italy.
2010-2013: Scientific director of the Protein Microsequencing Facility, San Raffaele Scientific Institute, Milan, Italy.
May 2013-present: Principal Investigator Senior, Functional Proteomics Group, FIRC Institute of Molecular Oncology Foundation (IFOM), Milan, Italy.
2002-2004: On-tenure Professor in “Proteomics”, Medical school, Vita-Salute San Raffaele University, Milan, Italy.
2004-2008: On-tenure Professor in “Bioinformatics in Genomics and Proteomics”, Biotechnology school, Vita-Salute San Raffaele University, Milan, Italy.
2009-2010: On-tenure Professor in “Proteomics and Structural Biology”, Biotechnology school, Vita-Salute San Raffaele University, Milan, Italy.
2010-present: On-tenure Professor in “Gene Expression and Proteomics”, Biotechnology school, Vita-Salute San Raffaele University, Milan, Italy.
2014: national scientific qualification as associate professor in Molecular Biology, General Biochemistry & Clinical Biochemistry and in Experimental Biology.
Brief statement of current research interests: Aim of the group is developing and applying mass spectrometry to biological and biomedical proteomics. We focussed our attention on different topics going from large scale organelles proteomics (RBC proteome, Synaptic vesicles proteome; Bachi A. Anal Chem 2008, Corti V. BMC Neurosci 2008) to the analysis of protein complexes (Diaz VM. Proteomics 2007, Bonnal S. Mol Cell. 2008, Bolis A. J Neurosci. 2009, Alpi E. et al. Biochem J. 2009, Panni S. Proteomics 2011), proteins post-translational modifications such as phosphorylation (Cursi S. EMBO J. 2006, Onofri F. J. Biol Chem. 2007, Magli A. J Biol Chem. 2010, Torta F.. J Proteome Res. 2009, Torta F. Methods Mol Biol. 2011), sumoylation (Matafora V. Mol Cell Proteomics 2009), redox state of cysteine-containing proteins (Anelli T. EMBO J. 2003, Camerini S. J Proteome Res. 2007, Torta F. Proteomics 2008, Torta F. Methods Enzymol. 2010, Torta F. Methods Mol Biol 2012), acetylation (Bonaldi T. EMBO J. 2003). Several efforts have been devoted to the developmennt of quantitative proteomics (Bachi A. J Proteomics 2008), for the study of global protein changes (Tasso R. Biomaterials 2012), quantitation of PTMs (Matafora V. Mol Cell Proteomics 2009, Torta F. Methods Mol Biol 2012), dynamics of protein-protein interactions (Gaetani M. Nucleic Acids Res. 2013) and for the identification of miRNA targets (Lechman ER. Cell Stem Cell 2013). The lab latest research takes advantages from recent technological innovations which have driven research in proteomics from single protein characterization to global approaches, aiming to achieve a comprehensive qualitative and quantitative description of complex molecular mechanisms. In particular we are interested in understanding how tumors tame the microenvironment to facilitate growth and spreading. To address this issue we exploit quantitative label free and SILAC based proteomics to characterize the molecular composition of patients derived cancer cells with the aim of identifying new prognostic factors able to better stratify patients and possibly also clarify the disease mechanisms. Second, we are studying the tumor-derived secretome, exosomes and microvescicles in order to identify specific molecular signatures in aggressive vs less aggressive tumor phenotypes and to elucidate which molecules prompt the microenvironment to an increased angiogenic and metastatic potential affecting the neighboring cells.
Principal recent publications :
- Soffientini P, Bachi A. STAGE-diging: A novel in-gel digestion processing for proteomics samples. J Proteomics. 2016 May 17;140:48-54.
- Aze A, Sannino V, Soffientini P, Bachi A, Costanzo V. Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression. Nat Cell Biol. 2016 Apr 25. doi: 10.1038/ncb3344.
- Poitelon Y, Bogni S, Matafora V, Della-Flora Nunes G, Hurley E, Ghidinelli M, Katzenellenbogen BS, Taveggia C, Silvestri N, Bachi A, Sannino A, Wrabetz L, Feltri ML. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun. 2015 Sep 18;6:8303.
- Matafora V, Cuccurullo M, Beneduci A, Petrazzuolo O, Simeone A, Anastasio P, Mignani R, Feriozzi S, Pisani A, Comotti C, Bachi A*, Capasso G. Early markers of Fabry disease revealed by proteomics. Mol Biosyst. 2015 May 19;11(6):1543-51.
- Matafora V, ZagatoL, FerrandiM, MolinariI, ZerbiniG, CasamassimaN, LanzaniC, Delli CarpiniS, TrepiccioneF, ManuntaP, Bachi* A, CapassoGB. Quantitative proteomics reveals novel therapeutic and diagnostic markers in hypertension. BBA Clinical 2 (2014) 79-87.
- Gregorc V, Novello S, Lazzari C, Barni S, Aieta M, Mencoboni M, Grossi F, De Pas T, de Marinis F, Bearz A, Floriani I, Torri V, Bulotta A, Cattaneo A, Grigorieva J, Tsypin M, Roder J, Doglioni C, Levra MG, Petrelli F, Foti S, Viganò M, Bachi A, Roder H. Predictive value of a proteomic signature in patients with non-small-cell lung cancer treated with second-line erlotinib or chemotherapy (PROSE): a biomarker-stratified, randomised phase 3 trial. Lancet Oncol. 2014 Jun;15(7):713-21.
- Cossetti C, Iraci N, Mercer TR, Leonardi T, Alpi E, Drago D, Alfaro-Cervello C, Saini HK, Davis MP, Schaeffer J, Vega B, Stefanini M, Zhao C, Muller W, Garcia-Verdugo JM, Mathivanan S, Bachi A, Enright AJ, Mattick JS, Pluchino S. Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells. Mol Cell. 2014 Sep 16. pii: S1097-2765(14)00675-3.
- Filosa G, Barabino SML, Bachi A. Proteomics Strategies to Identify SUMO Targets and Acceptor Sites: A Survey of RNA-Binding Proteins SUMOylation. Neuromolecular Med. 2013 Dec;15(4):661-76.
- Bachi A, Dalle Donne I, Scaloni A. Redox Proteomics: chemical principles, methodological approaches and biological/biomedical promises. Chem Rev. 2013 Jan 9;113(1):596-698.
* Corresponding author