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Upcoming Seminars

David Ron, MD - "Mechanisms that maintain protein folding homeostasis in the endoplasmic reticulum"

Professor of Cellular Pathophysiology and Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, England (UK)
When May 28, 2019
from 12:00 PM to 01:30 PM
Where Tigem, Vesuvius Auditorium
Contact Name
Contact Phone 081-19230659
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Abstract
A transcriptional and translational program that strives to match the complement of chaperones in the endoplasmic reticulum (ER) to the burden of unfolded proteins in the early secretory pathway (the Unfolded Protein Response or UPR) has long been known to exist and its functional importance has been showcased by genetic and pharmacological manipulations.
However, a homeostatic program centered solely on changes in the gene expression encounters an inherent latency imposed by the lag between changes in the internal milieu of the ER and rectifying changes in levels of UPR target mRNAs and proteins.
Here I will discuss how this problem might be resolved by post-translational adaptations that operate on a shorter time scale

Francesca Santoro, PhD - "Dimensionality explored in cell-material bioelectronics interfaces"

Italian Institute of Technology, Naples, Italy
When Jun 18, 2019
from 12:00 PM to 01:30 PM
Contact Name
Contact Phone 081-19230659
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Short CV

Abstract
The interface between biological cells and non-biological materials has profound influences on cellular activities, chronic tissue responses, and ultimately the success of medical implants and bioelectronic devices. For instance, electroactive materials in contact with cells can have very different composition, surface topography and dimensionality. Dimensionality defines the possibility to have planar (2D), pseudo-3D (planar with nano-micropatterned surface)1 and 3D conductive materials (i.e. scaffolds) in bioelectronics devices. Their success for both in vivo and in vitro applications lies in the effective coupling/adhesion of cells/tissues with the devices’ surfaces. It is known how a large cleft between the cellular membrane and the electrode surface massively affects the quality of the recorded signals or ultimately the stimulation efficiency of a device.
However, this field is hindered by lack of effective means to directly visualize in 3D cell-material interface at the relevant length scale of nanometers. In this work, we explored the use of ultra-thin plasticization technique2 to cells for the first time on materials which differ in dimensionality3, particularly focusing on the optimization of this procedure for 3D cell-materials interfaces which have been unexplored so far. We have characterized how cells differently elongate and deform their membranes in response to the dimensionality of the electroactive materials and the relevant processes at the biointerface. In this way, we are able to define a set of optimal conditions for cell-chip coupling which enable an appropriate approach for designing bioelectronics platforms for both in vivo and in vitro applications in 3 dimensions.
1 Santoro, F., Zhao, W., Joubert, L.-M., Duan, L., Schnitker, J., van de Burgt, Y., Lou, H.-Y., Liu, B., Salleo, A., Cui, L., Cui Y., Cui B., Revealing the Cell–Material Interface with Nanometer Resolution by Focused Ion Beam/Scanning Electron Microscopy. ACS Nano, 2017
2 Li X., Matino L., Zhang W., Klausen L., McGuire A., Lubrano C., Zhao W., Santoro F., Cui B., A nanostructure platform for live cell manipulation of membrane curvature, Nature Protocols, just accepted.
3 Iandolo D., Pennacchio F. A., Mollo V., Rossi D., Dannhauser D., Cui B., Owens R. M., Santoro F., Electron Microscopy for 3D Scaffolds–Cell Biointerface Characterization, Advanced Biosystems, 2018.