Cell Culture and Cytogenetics Core
The Cell Culture and Cytogenetics Core provides fully-equipped cell culture facilities and technical support for cellular and cytogenetic studies to TIGEM investigators.
In the last years, research at TIGEM has confirmed that collection of cell lines from individuals with genetic disorders is essential in order to test candidate genes and to perform functional studies. These cell lines have been collected and organized into a database, which includes a wide variety of immortalized cell lines, as well as fibroblast and lymphoblast cell lines from patients with various genetic diseases. This collection has been useful in providing readily available sources of DNA/RNA for projects organized for the investigation of pathogenetic mechanisms.
The Core is responsible for the establishment, maintenance, and centralized database storage of these cell lines. In addition, the Core offers cytogenetic services such as chromosome analyses (standard and high resolution chromosome analysis) and fluorescence in situ hybridization (FISH) on cell lines, peripheral blood samples, embryonic stem cells, induced pluripotent stem (iPS) cells, as well as directly on tissue samples. Lastly, the Core provides mouse primary hepatocytes cultures and performs screening and treatment processes for detection and removal of Mycoplasma contamination, respectively.