TRIM16	
EBBRHf  GGCGTAGAGTATGATACCATG 
EBBRHr	 TCCCATCACTGTAGCTGGCAA
	  		

PCR product 113 bp 

Designed on sequence 

PCR RUNNING CONDITIONS
95¡C x 10'
95¡C x 1' 
50¡C x 1' 
72¡C x 1'
35 cycles	

NAME            Chromosomal Assignment and Placement(s)
----            ---------------------------------------
trim16          Chromosome Chr17
                Places -47.32 cR from WI-9674 
                Places  33.97 cR from AFMA126YD5 (lod 2.35 relative to most  likely)

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